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Table 5 Commonly used vectors and unique restriction sites suitable for selectable marker restriction enzyme mediated gene disruption

From: Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

Vector Selectable marker Source Unique restriction sites in selectable marker
pUC18, pUC19 ampR New England Biolabs Xmn I, Sca I
pBlueScript ampR Stratagene Sca I
pZErO kanR Invitrogen Nco I
pBR322 ampR tetR New England Biolabs Sca I, Pvu I, Pst I Eco RV, Bam HI, Sph I, Sal I
pACYC177 ampR kanR New England Biolabs Sca I, Pst I, Bgl I Cla I, Hind III
pACYC184 camR tetR New England Biolabs Eco RI, Nco I Eco RV, Bam HI, Sph I, Sal I
  1. ampR : ampicillin resistance, kanR : kanamycin resistance, tetR : tetracycline resistance, camR : chloramphenicol resistance.