Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

BMC Research Notes

Table 5 Commonly used vectors and unique restriction sites suitable for selectable marker restriction enzyme mediated gene disruption

Vector	Selectable marker	Source	Unique restriction sites in selectable marker
pUC18, pUC19	amp^R	New England Biolabs	Xmn I, Sca I
pBlueScript	amp^R	Stratagene	Sca I
pZErO	kan^R	Invitrogen	Nco I
pBR322	amp^R tet^R	New England Biolabs	Sca I, Pvu I, Pst I Eco RV, Bam HI, Sph I, Sal I
pACYC177	amp^R kan^R	New England Biolabs	Sca I, Pst I, Bgl I Cla I, Hind III
pACYC184	cam^R tet^R	New England Biolabs	Eco RI, Nco I Eco RV, Bam HI, Sph I, Sal I

amp^R : ampicillin resistance, kan^R : kanamycin resistance, tet^R : tetracycline resistance, cam^R : chloramphenicol resistance.

ISSN: 1756-0500