Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say)

Table 1 A list of primers of 9 candidate house-keeping genes for RT-PCR

Gene name^a	Primer sequences (5’to 3’)		Amplicon size (bp)	Accession no. of homolog^b
ACT1	Forward	GTGAGCAGTGTCCAACCTC	548	XP_966495
	Reverse	GGGAAGAGCGTAACCTTCG	548	XP_966495
ACT2	Forward	GAGAAGATGGCGCAGATCATG	720	NP_001165843
	Reverse	CCACATCTGTTGGAACGTGG	720	NP_001165843
ARF1	Forward	GGGGAGGCAAACCGGTCA	603	XP_973025
	Reverse	GGCTTCTAAACCTAGTGCCTGG	603	XP_973025
ARF4	Forward	GGACCTATCTTCAGCTATGCGT	681	XP_970752
	Reverse	CAATCCCTCGTGAAGGCCA	681	XP_970752
EF1α	Forward	CCTTGTTCTGGGCAAACAGG	192	XP_968773
	Reverse	AACCTTGCCCATCAGCAC	192	XP_968773
RP4	Forward	GCACCAGGTCTTGTTCGTG	311	XP_971634
	Reverse	GGGGAATACGGGCGACAG	311	XP_971634
RP18	Forward	ACTTCGTGTCACTGAAACTGC	252	XP_968042
	Reverse	TATCCGCACGACTTCCTGC	252	XP_968042
TBP1	Forward	ATAACCCTGGCCGTCTCCATG	886	XP_969256
	Reverse	TGTACTGTCGCCCGGGTTGAAC	886	XP_969256
TBP2	Forward	TACGAGAACCCCGTACCACT	345	XP_969326
	Reverse	GCCTAACTTTGGCACCCGT

a: ACT1 and ACT2, Actin; ARF1 and ARF4, ADP-ribosylation factor; TBP1 and TBP2, TATA box binding protein; RP4 and RP18, ribosomal protein; EF1α, translation elongation factor 1α. They are named according to the corresponding genes in the T. castaneum genome. b: the accession number of homologs in T. castaneum.

ISSN: 1756-0500