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Table 2 Primers of 9 candidate house-keeping genes used in qRT-PCR

From: Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say)

Gene namea

Primer sequenceb

Amplicon size (bp)

Slopec

R2d

Efficiencye

ACT1

F-CAAAGCCAACAGGGAGAAGATGAC

105

−3.131

0.991

2.086

R-CGACCAGAAGCGTACAAGGAGAG

ACT2

F- TTCTGATTCCGTGAGGATTTTG

149

−3.313

0.985

2.004

R- GTGAGGTGGATGTTCGTAGGG

ARF1

F- CGGTGCTGGTAAAACGACAA

135

−3.131

0.964

2.086

R- TGACCTCCCAAATCCCAAAC

ARF4

F- GTGCTCGTGAACCATGTGAA

140

−3.130

0.997

2.087

R- AACCTCCAATCCCTCGTGAA

EF1α

F- CAGGGCAAGGTTTGAAAGATAA

111

−3.216

0.972

2.046

R- CGTCTGCTTTGCGATTGAG

RP4

F- AAAGAAACGAGCATTGCCCTTCCG

119

−3.317

0.984

2.002

R- TTGTCGCTGACACTGTAGGGTTGA

RP18

F- TAGAATCCTCAAAGCAGGTGGCGA

133

−3.158

0.976

2.073

R- AGCTGGACCAAAGTGTTTCACTGC

TBP1

F- ATGTCAAGCAGAAAGTCAAGAATCC

173

−3.204

0.988

2.054

R- GCCGTAATATCCCTAACTCCCAAG

TBP2

F- AGCGAGGAAGACTCCAGGTTG

171

−3.018

0.957

2.144

 

R- ACTACTGAAAGAACTGTGAGTGAGC

    
  1. a: ACT1 and ACT2, Actin; ARF1 and ARF4, ADP-ribosylation factor; TBP1 and TBP2, TATA box binding protein; RP4 and RP18, ribosomal protein; EF1α, translation elongation factor 1α. b, Primer sequence of RP4 and RP18 were adopted from Zhu et al. (2011)[29]. c, Slope value of the standard curve. d, Regression coefficient calculated from the regression line of the standard curve. e, Real-time qRT-PCR efficiency calculated by the standard curve method.