Factor

ARF4

ARF1

RP4

RP18

BK(n = 4)

BK(n = 3)

BK(n = 2)


n

21

21

21

21

21

21

21

GM[CP]

23.59

21.62

19.34

16.81

20.18

19.16

19.07

AM[CP]

23.62

21.64

19.37

16.83

20.2

19.18

19.09

min [CP]

21.41

19.67

17.27

15.36

18.28

17.35

17.38

max [CP]

25.24

22.99

20.96

18.16

21.23

20.21

20.43

SD[±CP]

1.13

0.75

0.83

0.60

0.77

0.71

0.65

CV[%CP]

4.79

3.48

4.29

3.59

3.82

3.69

3.41

min[xfold]

−4.96

−4.2

−4.2

−2.88

3.83

3.6

3.29

max[xfold]

3.38

2.73

3.09

2.68

2.1

2.1

2.63

SD[±xfold]

2.25

1.72

1.82

1.54

1.73

1.65

1.59

 Descriptive statistics of four HKGs based on their crossing point (CP) values. Abbreviations: n: number of samples, GM [CP] The geometric mean of CP, AM [CP], The arithmetic mean of CP, min [CP] and max [CP] The extreme values of CP, SD [±CP] The standard deviation of the CP CV [%CP] The coefficient of variance expressed as a percentage on the CP level, min [xfold] and max [xfold] The extreme values of expression levels expressed as an absolute xfold over or underregulation coefficient, SD [± xfold] Standard deviation of the absolute regulation coefficients. Results from three independent experiments are shown. In the three last columns the BestKeeper index is computed together with the same descriptive parameters, for four genes (ARF4, ARF1, RP4 and RP18) or for three genes after removal of ARF4 (ARF1, RP4 and RP18) or for two genes after removal of RP4 (ARF1 and RP18).