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Table 2 Sequencing statistics for the studied samples

From: False positives complicate ancient pathogen identifications using high-throughput shotgun sequencing

Platform

Sample Description

No. Filtered Sequences

Molecular Length (bp)

Total Sequenced (bp)

HeliScope

Grand Plaza Bulk 1

31,183,210

34.1 ± 7.1

1,063,972,634

Grand Plaza Bulk 2

30,773,387

34.4 ± 7.2

1,058,435,915

Grand Plaza Phos. 1

26,269,497

34.4 ± 7.3

904,192,902

Grand Plaza Phos. 2

23,061,503

35.0 ± 7.4

806,304,129

Churchyard Bulk 1

28,960,308

33.9 ± 7.0

981,718,381

Churchyard Bulk 2

27,035,110

33.8 ± 7.0

914,002,286

Churchyard Phos. 1

28,423,683

34.5 ± 7.3

981,600,068

Churchyard Phos. 2

29,660,255

33.8 ± 7.0

1,003,204,069

Grand Plaza Sheared

4,088

27.1 ± 3.4

110,619

Churchyard Sheared

5,309

27.2 ± 3.5

144,140

Soil Bulk

840,915

33.4 ± 7.2

28,082,034

Soil Sheared

123,211

31.4 ± 6.4

3,869,834

HiSeq 2500

TP04

9,643,548

219.5 ± 43.9

2,117,000,413

 

TP09

6,640,789

236.2 ± 39.2

1,568,449,339

 

TP10

5,368,267

223.7 ± 40.3

1,201,119,486

 

TP18

7,693,734

215.5 ± 39.1

1,658,247,428

 

TP37

9,113,392

207.2 ± 39.0

1,888,081,563

 

TP48

3,720,696

227.8 ± 40.5

847,609,750

 

Soil Bulk

4,807,489

222.3 ± 40.6

1,068,497,871

  1. “Bulk” samples are the unsheared bulks. “Phos.” samples are the phosphatase-treated unsheared bulks. “Sheared” samples are the sheared bulks. The “No. Filtered Sequences” is the number of sequences after filtering for sequencing artifacts (e.g. PCR duplicates). “Molecular Length” is the mean length (± standard deviation) of the generated reads.