Table 2 Analyzed murine genes and their specific real-time QPCR conditions
Mouse genes | Accession number (NCBI) | Oligonucleotides (sense/antisense) | Amplicon length (bp) | QPCR conditionsa | |
|---|---|---|---|---|---|
Ann. T (°C) | Acq. T (°C) | ||||
Cyp11a1 | NM_019779 | ATCCGGGCTTCTTTCCCAATC/GGATGGGGTTCTCAGGCATC | 249 | 64 | 86 |
Hsd3b1 | NM_008293 | TGCCAGGGCATCTCTGTTGTC/TCTGTTCCTCGTGGCCATTCA | 220 | 64 | 84 |
Cyp21a1 | NM_009995 | TCACGACTGTGTCCAGGACTTG/TTCGTCTTTGCCATCCCTTTG | 250 | 67 | 84 |
Akr1C18 | NM_1340662.2 | GCACCATAGGCAACCAGAAC/TCTCATTCATTTCCCAGTGTCTC | 312 | 54 | 78 |
Cyp11b1 | NM_001033229 | CTGGGACAGTCCTCAATGTGA/ATCCGCACATCCTCTTTCTCTT | 244 | 62 | 87 |
Hsd11b1 | NM_008288 | GGCCAGCAAAGGGATTGGAAG/TTTTCCCAGCCAAGGAGGAGA | 401 | 66 | 85 |
Hsd11b2 | NM_008289 | TGGCTGACGTGGGACTGTCT/TTGGAGCAGCCAGGCTTGATA | 277 | 63 | 87 |
Gapdh | NM_008084 | GTCGGTGTGAACGGATTTG/AAGATGGTGATGGGCTTCC | 215 | 61 | 84 |
Hmbs | NM_013551 | GGAATGCATGTATGCTGTGGG/CAGGTACAGTTGCCCATCTT | 208 | 59 | 85 |