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Table 2 PCR conditions for BCR-ABL1

From: Phase distribution of chronic myeloid leukemia in Bangladesh

Hot-start PCR 15 min 95°C
3-step cycling   
Denaturation 30 s 94°C
Annealing 30 s 55°C
Extension 1 min 72°C
Number of cycles 30  
Final extension 1 min 72°C