Table 2 PCR conditions for BCR-ABL1
From: Phase distribution of chronic myeloid leukemia in Bangladesh
| Hot-start PCR | 15 min | 95°C |
|---|---|---|
| 3-step cycling | ||
| Denaturation | 30 s | 94°C |
| Annealing | 30 s | 55°C |
| Extension | 1 min | 72°C |
| Number of cycles | 30 | |
| Final extension | 1 min | 72°C |