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Table 2
PCR conditions for BCR-ABL1
From:
Phase distribution of chronic myeloid leukemia in Bangladesh
Hot-start PCR
15 min
95°C
3-step cycling
Denaturation
30 s
94°C
Annealing
30 s
55°C
Extension
1 min
72°C
Number of cycles
30
Final extension
1 min
72°C
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