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Figure 2 | BMC Research Notes

Figure 2

From: Analysis options for high-throughput sequencing in miRNA expression profiling

Figure 2

qPCR validation of miRNA profiling using HTS and microarray data. Profiling of 6 miRNAs using microarray analysis, qPCR and results of three different HTS analysis pipelines (A-C) shows a very similar expression pattern. Box plots present distribution of appropriately normalized miRNA expression in 20 samples. The seed sequence for pipeline C is derived from the miRBase entry sequence of the respective miRNA. Best concordance of miRNA abundance is seen between microarray data (D), qPCR from cDNA (E), qPCR from sequencing library DNA (F) and HTS data analysis pipeline C (C). miRNAs were labeled according to the key: L1 – hsa-miR-27b-5p, L2 - hsa-miR-708-3p, M1 – hsa-miR-30e-5p, M2 – hsa-miR-146a-5p, H1 – hsa-miR-181a-5p, H2 – hsa-miR-143-3p.

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