PCR amplification of JSRV env and gag sequences from genomic DNA. (A) Representative image of PCR products amplified using env- (upper panel), gag- (middle panel) and GAPDH-specific (lower panel) primers. Lanes 1; water only, 2; lung cancer tissue array - first round PCR, 3; lung cancer tissue array - second round PCR, 4; HBE135-E6E7, 5; A549, 6; nasopharyngeal cancer tissue array, 7; molecular weight marker, 8; non-neoplastic human lung, 9; OPA lung tumor cell line, JS7, and 10; normal sheep lung. Nucleotide (B) and amino acid (C) sequence alignment of PCR products amplified with env-specific primers. Nucleotide (D) and amino acid (E) sequence alignment of PCR products amplified with gag-specific primers. AF105220; accession number of the full-length JSRV genome deciphered by Palmarini et al. , AF357971; accession number of the full-length JSRV genome deciphered by De Martini et al. , AF153615; accession number of an endogenous JSRV genome deciphered by Palmarini et al. , EF680297 accession number of an endogenous JSRV deciphered by Arnaud et al. , DQ838494; accession number of a full-length JSRV genome of Chinese origin, TA; tissue array, Biopsy 9; adenocarcinoma in-situ #9.