Figure 3
Analysis of the growth of wild-type, spy1236 knockout and complemented strains cultured in acidic media (pH 6.0) in an atmosphere containing 5% CO 2 . The experiment was performed as described in Figures 1 and 2. At least three independent experiments were performed and they always yielded essentially the same results. The error bars indicate the standard error of the mean. (a) The CFU/ml broth culture of wild-type strain MDYK and its derived strain MDYKΔspy1236 are shown. (b) The CFU/ml culture of MDYKΔspy1236 (pLZ-spy1236) and MDYKΔspy1236 (pLZ12-Km2) grown in a broth supplemented with 62.5 mg/mL kanamycin are shown. Viable counts were performed on BHI-Y agar plates supplemented with 125 mg/mL kanamycin. (c) The CFU/ml broth culture of wild-type strain MDN and its derived strain MDNΔspy1236 are shown. (d) The CFU/ml culture of MDNΔspy1236 (pLZ-spy1236) and MDNΔspy1236 (pLZ12-Km2) grown in a broth supplemented with 62.5 mg/mL kanamycin are shown. Viable counts were performed on BHI-Y agar plates supplemented with 125 mg/mL kanamycin.