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Table 1 Sequences of primers used in qRT-PCR

From: Relevance of the two-component sensor protein CiaH to acid and oxidative stress responses in Streptococcus pyogenes

Primer

Sequence (5′-3′)

GyrA-1584 F

ACGTGGCGTCCAAGGAACT

GyrA-1709R

TGCTAAGCTTTCAACCGATAGACA

HtrA-F4

ATCGACGGAGCTAAACGAATTG

HtrA-R4

TCAGCTCCAACTAATTCACCAACA

Nrd-F

ACAGTAGACAAGCTGAAGACGGC

Nrd-R

AGCGAGTATGACACTGTTCACATTC

Emm1-31 F

TGCTACTCCAGCTGTTGCCATA

Emm1-98R

ACAGGTGAAACAGCTAACCCATTC

MGAS5005 polA-F

GCGGGCAAAACCACCTT

MGAS5005 polA-R

GCGCGACCCGCCTTATA

  1. Primers were designed by using Primer Express® software version 3.0 and 2.0, and with technical support of life technologies Japan.
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BMC Research Notes

ISSN: 1756-0500

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