Skip to main content

Table 3 Distribution of NGS reads based on Cq and delta Cq values

From: A detailed analysis of next generation sequencing reads of microRNA expression in Barrett’s Esophagus: absolute versus relative quantification

Table 3a

Table 3b

Cq values

Average NGS reads

delta Cq values

Average NGS reads

<20

54996

<0

50428

20-24

75764

0-4

30414

25-29

2621

5-9

1485

30-34

2466

10-14

955

35-39

383

15-19

435

  1. NGS, next generation sequencing.
  2. Cq, threshold value on qRT-PCR.
  3. delta Cq, Cq(miRNA)-Cq(RNU6B).
Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us