Figure 4
From: Identification and enzymatic characterization of acid phosphatase from Burkholderia gladioli
pH sensitivity of the catalysis of membrane-bound acid phosphatase from Burkholderia gladioli . Enzymatic assays containing 1 mM PNPP were buffered with 50 mM acetate for the pH range 3.5-6.5, and 50 mM Tris–HCl for the pH range 6.5-8.0. There was no significant difference between the two buffers used at pH 6.5. The pH before and after each determination did not differ by more than 0.05 units, and the reaction was initiated by the addition of membrane-bound enzyme.