Skip to main content

Table 1 Relative effectiveness of several reagents on the activity of membrane-bound acid phosphatase from the R 3.25 isolate

From: Identification and enzymatic characterization of acid phosphatase from Burkholderia gladioli

Reagent

Residual activity

Phosphate (10 mM)

38

EDTA (10 mM)

95

Arsenate (1 mM)

14

Magnesium (2 mM)

96

Calcium (1 mM)

94

Zinc (1 mM)

97

Cobalt (1 mM)

94

Levamisole (10 mM)

93

Sodium tartrate (10 mM)

95

Bafilomycin A1 (1 mM)

98

Oligomycin (1.5 mg/ml)

93

Ouabain (1.3 mM)

92

Pantoprazol (6 mM)

95

PHMB (1 mM)

5

Vanadate (0.5 mM)

6

  1. Specific activity of 100% corresponds to 109.27 U/mg.