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Figure 2 | BMC Research Notes

Figure 2

From: Butyrate induced changes in Wnt-signaling specific gene expression in colorectal cancer cells

Figure 2

Overexpression of DN-Tcf4 in induced HCT-116 cells. A stably transfected clone of HCT-116 cells was treated with 4 μg/ml doxycycline (Doxy) and/or 5 mM NaB for 17.5 hr or left untreated. Protein was isolated, and DN-Tcf4 detected with anti-FLAG antibody in western blot analysis. Actin was analyzed as a control. Duplicate samples from a representative western blot shown.

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