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Table 1 Murine Sepsis Score (MSS) to assess the severity of disease in an experimental model of fecal-induced peritonitis

From: A robust scoring system to evaluate sepsis severity in an animal model

Variable Score and description
Appearance 0- Coat is smooth
1- Patches of hair piloerected
2- Majority of back is piloerected
3- Piloerection may or may not be present, mouse appears “puffy”
4- Piloerection may or may not be present, mouse appears emaciated
Level of consciousness 0- Mouse is active
1- Mouse is active but avoids standing upright
2- Mouse activity is noticeably slowed. The mouse is still ambulant.
3- Activity is impaired. Mouse only moves when provoked, movements have a tremor
4- Activity severely impaired. Mouse remains stationary when provoked, with possible tremor
Activity 0- Normal amount of activity. Mouse is any of: eating, drinking, climbing, running, fighting
1- Slightly suppressed activity. Mouse is moving around bottom of cage
2- Suppressed activity. Mouse is stationary with occasional investigative movements
3- No activity. Mouse is stationary
4- No activity. Mouse experiencing tremors, particularly in the hind legs
Response to stimulus 0- Mouse responds immediately to auditory stimulus or touch
1- Slow or no response to auditory stimulus; strong response to touch (moves to escape)
2- No response to auditory stimulus; moderate response to touch (moves a few steps)
3- No response to auditory stimulus; mild response to touch (no locomotion)
4- No response to auditory stimulus. Little or no response to touch. Cannot right itself if pushed over
Eyes 0- Open
1- Eyes not fully open, possibly with secretions
2- Eyes at least half closed, possibly with secretions
3- Eyes half closed or more, possibly with secretions
4- Eyes closed or milky
Respiration rate 0- Normal, rapid mouse respiration
1- Slightly decreased respiration (rate not quantifiable by eye)
2- Moderately reduced respiration (rate at the upper range of quantifying by eye)
3- Severely reduced respiration (rate easily countable by eye, 0.5 s between breaths)
4- Extremely reduced respiration (>1 s between breaths)
Respiration quality 0- Normal
1- Brief periods of laboured breathing
2- Laboured, no gasping
3- Laboured with intermittent gasps
  4- Gasping