Array analysis of PCR products with similar overall length but different 5′ segment lengths: probe set 2. Panels show the signal intensity for perfect match and mismatch probes hybridised to PCR products with total lengths between 289 and 317 bases and 5′ segment lengths ranging from 23 to 246 bases; each panel shows the effect of a different mismatch at three positions within the probe. Approximately 1012 copies of PCR amplicon were applied to each array. Results shown are mean values for duplicate spots on the same array (error bars show standard deviation). 5AA = AA mismatch between probe and PCR product located in the 5′ third of the probe, etc.