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Figure 4 | BMC Research Notes

Figure 4

From: Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array

Figure 4

Array analysis of PCR products with the same 5′ segment length but different total lengths. Arrays were hybridised with PCR products with constant 5′ segment lengths (A: 43 bases; B: 191 bases; C: 272 bases) but different overall lengths ranging from 94 to 978 bases, 242 to 1126 bases, and 323 to 1207 bases, respectively. Signal intensities were normalised relative to the signal for the perfect match probe on each array and are plotted against the 5′ segment length shown as a proportion of the total product length. Approximately 1012 copies of PCR amplicon were applied to each array. Results shown are mean values for duplicate spots on the same array (error bars show standard deviation). PM = perfect match probe. AA = AA mismatch between probe and PCR product, etc.

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