Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array

Table 2 Primers used for biotin-labelled PCR

Forward primer	Forward primer sequence (5′ to 3′)	Reverse primer	Reverse primer sequence (5′ to 3′)	Amplicon length (bases)	Length of 5′ segment (bases)
for1	CTGGAGGAGCCTTCCCTAC	rev1	GTCGCGACTGCCGCTC	94	43
for2	CCTCGCATTGCTCAAGCACA	rev1	as above	145	43
for3	CGCGACACGCCCATGT	rev1	as above	243	43
for4	GCGGACATCGACGGCTATGT	rev1	as above	312	43
for4 lim*	AACGCGCGGACATCGACGGCTATGT	rev1	as above	317	43
for5	GCGCATACGATTCTTCAAAAGGC	rev1	as above	978	43
for3	as above	rev2	ACGCCTTACGTATCGGATCG	298	107
for6	CTTCGGGCCACCGCTACTA	rev3	TGTGCTGTGGGCCGTGCT	300	149
for1	as above	rev4	TCACCGCGCCTTGCTCGT	242	191
for2	as above	rev4	as above	293	191
for3	as above	rev4	as above	382	191
for7	as above	rev4	as above	624	191
for5	as above	rev4	as above	1126	191
for1	as above	rev5	CCTCGGGCGTTTCGATCAC	323	272
for4	as above	rev5	as above	541	272
for8	as above	rev5	as above	878	272
for5	as above	rev5	as above	1027	272
for8	CCAATGCATGTCGGCTCGC	rev6	ACACCTCTTGCACGGAACCG	296	23**
for9	GACACAAGCCGCGAACTGAC	rev7	CCCAATGTCCGACATAGCC	295	95**
for10	GAGCATCTTCGCGCCATAG	rev8	TGTCGCGCTCGTTCGCTG	289	149**
for7	CCTTTGCCGTCAGCTTCCG	rev9	TAGTAGCGGTGGCCCGAAG	301	201**
for11	CAGTTGTCCCTGAAGCGCCT	rev10	TTGAGCAATGCGAGGCTGC	317	246**

ISSN: 1756-0500