Skip to main content

Table 3 Limit of detection of PCR products with similar total lengths but different 5′ segment lengths

From: Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array

  

Signal intensity (mean ± sd)*

Total length (bases)

5′ segment length (bases)

Approx. 8 × 109copies

Approx. 8 × 108copies

Approx. 8 × 107copies

312

43

0.63 ± 0.03

0.23 ± 0.03

-

323

272

0.62 ± 0.01

0.22 ± 0.02

-

296

23

0.66 ± 0.06

0.28 ± 0.05

-

317

246

0.57 ± 0.03

0.24 ± 0.00

-

  1. *Results shown are mean values for duplicate spots on the same array; -: negative.