Skip to main content

Table 3 Limit of detection of PCR products with similar total lengths but different 5′ segment lengths

From: Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array

   Signal intensity (mean ± sd)*
Total length (bases) 5′ segment length (bases) Approx. 8 × 109copies Approx. 8 × 108copies Approx. 8 × 107copies
312 43 0.63 ± 0.03 0.23 ± 0.03 -
323 272 0.62 ± 0.01 0.22 ± 0.02 -
296 23 0.66 ± 0.06 0.28 ± 0.05 -
317 246 0.57 ± 0.03 0.24 ± 0.00 -
  1. *Results shown are mean values for duplicate spots on the same array; -: negative.
\