Figure 2

Activation of the canonical Wnt-pathway by GSK3 inhibition. (A, B) Assessment of the Wnt/beta-catenin pathway activity after exposure to the GSK3 inhibitors BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM) or CHIR-98014 (1 μM) in (A) ES-D3 cells and (B) ES-CCE cells using the M50 Super 8x TOPflash luminescence reporter system. A M51 Super 8x FOPflash vector with mutated TCF/LEF binding sites was used as a negative control. As a positive control, the ES cells were incubated with 50 ng/ml Wnt3a. Values are means of the relative light units of the firefly and renilla luciferase luminescence ratio ± SEM, n = 6. ** p < 0.01, * p < 0.05 compared to randomly treated cells, ## < 0.01, # < 0.05 Wnt3a treated cells, ANOVA with Dunnett’s post hoc test.