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Figure 3 | BMC Research Notes

Figure 3

From: Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors

Figure 3

Gene expression changes upon treatment with GSK3 inhibitors. (A-C) qPCR analysis of T and the pluripotency markers Nanog and Pou5F1 after a six day treatment of mouse ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Randomized differentiation without any growth factors or small molecules was used as a control. Values are calibrated normalized relative quantities (CNRQ) after normalization to three stably expressed housekeeping genes and scaling to undifferentiated ES cells. Values are means ± SEM, n = 4–5. ** p < 0.01, * p < 0.05 compared to randomly differentiated ES cells of the same day, ANOVA with Dunnett’s post hoc test.

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