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Table 1 Exclusion criteria used for filtering spurious trajectories of SM-Atto647N diffusing on a mica-supported DOPC bilayer at 25°C

From: TrackArt: the user friendly interface for single molecule tracking data analysis and simulation applied to complex diffusion in mica supported lipid bilayers

Exclusion criterion Value
Min frames number 20
Min D (μm2/s) 0.1
Max D (μm2/s) 10
Min individual MSD fit R2 0.9
Min average trajectory intensity 0.3
Max average trajectory intensity 1.5