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Table 1 Kinetic parameters obtained by nonlinear least-squares global fitting of data from Figures 2 and 3

From: Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD+-dependent DNA ligase from Haemophilus influenzae

Parameter

Value from NMN study

Value from AMP study

Vmax

150 ± 15 nmol/min/mg

169 ± 13 nmol/min/mg

KmA

650 ± 80 nM

410 ± 50 nM

KmB

62 ± 9 nM

85 ± 9 nM

Ki

66 ± 14 nM

32 ± 8 nM

Kia

610 ± 120 nM

---

Kip

3.3 ± 0.4 μM

---

Kir

---

390 ± 60 μM

  1. KmA and KmB are the Michaelis constants for NAD+ and nDNA, respectively. Ki is the dissociation constant for nDNA with unadenylylated DNA ligase (i.e. substrate inhibition constant for nDNA). Kia is the dissociation constant for NAD+ with unadenylylated DNA ligase. Kip is the dissocation constant for NMN. Kir is the dissociation constant for AMP. Standard errors of best-fit values are shown.
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BMC Research Notes

ISSN: 1756-0500

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