|
Parameter
|
Value from NMN study
|
Value from AMP study
|
|---|
|
Vmax
|
150 ± 15 nmol/min/mg
|
169 ± 13 nmol/min/mg
|
|
KmA
|
650 ± 80 nM
|
410 ± 50 nM
|
|
KmB
|
62 ± 9 nM
|
85 ± 9 nM
|
|
Ki
|
66 ± 14 nM
|
32 ± 8 nM
|
|
Kia
|
610 ± 120 nM
|
---
|
|
Kip
|
3.3 ± 0.4 μM
|
---
|
|
Kir
|
---
|
390 ± 60 μM
|
- KmA and KmB are the Michaelis constants for NAD+ and nDNA, respectively. Ki is the dissociation constant for nDNA with unadenylylated DNA ligase (i.e. substrate inhibition constant for nDNA). Kia is the dissociation constant for NAD+ with unadenylylated DNA ligase. Kip is the dissocation constant for NMN. Kir is the dissociation constant for AMP. Standard errors of best-fit values are shown.
