Figure 3
Validation of candidate reference genes in Barnea, Frantoio and Picual independently using GeNorm algorithm in qBase Plus. (A) Average expression stability values (M) of the eight reference genes plotted from least stable (left) to most stable (right) in Barnea (A), Frantoio (C) and Picual (E) (B) Pairwise variation analysis (Vn/Vn+1) between the normalisation factors NFn and NFn + 1 to determine the optimal number of reference genes to be used for normalisation against target genesin Barnea (B), Frantoio (D) and Picual (F).