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Table 3 BestKeeper descriptive statistical analyses of eight reference genes in Barnea, Frantoio and Picual mesocarp together (A) and individually (B, C and D)

From: Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real-time RT-PCR

Factors Stability of reference genes in Barnea, Frantoio and Picual (A)
  60S RBP L18-3 PP2A PTB GAPDH EF1-alpha OUB2 TIP2 TUBA
n 24 24 24 24 24 24 24 24
SD [±Cq] 0.9 1 0.93 0.94 0.99 1.07 2.64 1.54
CV [% Cq] 2.87 3.08 2.69 3.29 4.26 4.41 7.66 5.11
r value 0.654 0.805 0.683 0.717 0.769 0.711 0.832 0.665
p value 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001
Ranking 5 1 4 3 2 7 6 8
  Stability of reference genes in Barnea (B)
n 8 8 8 8 8 8 8 8
SD [±Cq] 1.51 0.94 1.56 0.99 0.98 1.00 3.50 1.50
CV [% Cq] 4.86 3.49 4.56 4.31 4.29 4.45 10.12 4.91
r value 0.744 0.886 0.720 0.796 0.854 0.771 0.866 0.811
p value 0.034 0.003 0.044 0.039 0.007 0.023 0.005 0.014
Ranking 5 1 6 3 2 4 7 8
  Stability of reference genes in Frantoio (C)
n 8 8 8 8 8 8 8 8
SD [±Cq] 0.53 0.47 0.26 0.68 0.44 0.28 1.15 1.38
CV [% Cq] 1.68 1.46 0.75 2.36 1.95 1.19 3.32 4.56
r value 0.778 0.686 0.713 0.835 0.827 0.796 0.510 0.682
p value 0.004 0.021 0.032 0.010 0.004 0.020 0.003 0.002
Ranking 4 6 5 1 2 3 8 7
  Stability of reference genes in Picual (D)
n 8 8 8 8 8 8 8 8
SD [±Cq] 0.36 0.53 0.83 0.98 0.99 0.96 3.32 1.51
CV [% Cq] 1.13 1.64 2.38 2.56 2.80 2.42 9.66 5.04
r value 0.969 0.883 0.659 0.797 0.809 0.946 0.867 0.753
p value 0.001 0.004 0.015 0.018 0.015 0.001 0.005 0.031
Ranking 1 3 6 5 4 2 7 8
  1. Abbreviations: n number of samples, SD [±Cq] standard deviation of Cq values, CV [% Cq] coefficient of variance expressed as percentage of Cq values, r coefficient of correlation, p probability value. Genes showing the highest r value and genes with SD > 1 have been highlighted in bold. Ranking of the eight reference genes based on their r-value and SD values have also been shown.