|
Factors
|
Stability of reference genes in Barnea, Frantoio and Picual (A)
|
|---|
| |
60S RBP L18-3
|
PP2A
|
PTB
|
GAPDH
|
EF1-alpha
|
OUB2
|
TIP2
|
TUBA
|
|---|
|
n
|
24
|
24
|
24
|
24
|
24
|
24
|
24
|
24
|
|
SD [±Cq]
|
0.9
|
1
|
0.93
|
0.94
|
0.99
|
1.07
|
2.64
|
1.54
|
|
CV [% Cq]
|
2.87
|
3.08
|
2.69
|
3.29
|
4.26
|
4.41
|
7.66
|
5.11
|
|
r value
|
0.654
|
0.805
|
0.683
|
0.717
|
0.769
|
0.711
|
0.832
|
0.665
|
|
p value
|
0.001
|
0.001
|
0.001
|
0.001
|
0.001
|
0.001
|
0.001
|
0.001
|
|
Ranking
|
5
|
1
|
4
|
3
|
2
|
7
|
6
|
8
|
| |
Stability of reference genes in Barnea (B)
|
|
n
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
|
SD [±Cq]
|
1.51
|
0.94
|
1.56
|
0.99
|
0.98
|
1.00
|
3.50
|
1.50
|
|
CV [% Cq]
|
4.86
|
3.49
|
4.56
|
4.31
|
4.29
|
4.45
|
10.12
|
4.91
|
|
r value
|
0.744
|
0.886
|
0.720
|
0.796
|
0.854
|
0.771
|
0.866
|
0.811
|
|
p value
|
0.034
|
0.003
|
0.044
|
0.039
|
0.007
|
0.023
|
0.005
|
0.014
|
|
Ranking
|
5
|
1
|
6
|
3
|
2
|
4
|
7
|
8
|
| |
Stability of reference genes in Frantoio (C)
|
|
n
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
|
SD [±Cq]
|
0.53
|
0.47
|
0.26
|
0.68
|
0.44
|
0.28
|
1.15
|
1.38
|
|
CV [% Cq]
|
1.68
|
1.46
|
0.75
|
2.36
|
1.95
|
1.19
|
3.32
|
4.56
|
|
r value
|
0.778
|
0.686
|
0.713
|
0.835
|
0.827
|
0.796
|
0.510
|
0.682
|
|
p value
|
0.004
|
0.021
|
0.032
|
0.010
|
0.004
|
0.020
|
0.003
|
0.002
|
|
Ranking
|
4
|
6
|
5
|
1
|
2
|
3
|
8
|
7
|
| |
Stability of reference genes in Picual (D)
|
|
n
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
8
|
|
SD [±Cq]
|
0.36
|
0.53
|
0.83
|
0.98
|
0.99
|
0.96
|
3.32
|
1.51
|
|
CV [% Cq]
|
1.13
|
1.64
|
2.38
|
2.56
|
2.80
|
2.42
|
9.66
|
5.04
|
|
r value
|
0.969
|
0.883
|
0.659
|
0.797
|
0.809
|
0.946
|
0.867
|
0.753
|
|
p value
|
0.001
|
0.004
|
0.015
|
0.018
|
0.015
|
0.001
|
0.005
|
0.031
|
|
Ranking
|
1
|
3
|
6
|
5
|
4
|
2
|
7
|
8
|
- Abbreviations: n number of samples, SD [±Cq] standard deviation of Cq values, CV [% Cq] coefficient of variance expressed as percentage of Cq values, r coefficient of correlation, p probability value. Genes showing the highest r value and genes with SD > 1 have been highlighted in bold. Ranking of the eight reference genes based on their r-value and SD values have also been shown.
