Validation of the ChIP-seq library construction method by ChIP-seq against macroH2A1.2. (A) Bioanalyzer 2100 gel image showing 1 ul of each library run on DNA 1000 chip (B) Validation of several macroH2A1.2 peaks by qPCR at the respective loci. Three loci not bound by macroH2A1 are on the left side (red) and eight loci identified as macroH2A1 peaks are on the right side (green). Loci are named according to their location on the mm9 mouse genome build. (C) A UCSC genome browser screen shot of representative macroH2A1.2 peaks. Input DNA is colored in blue and the two anti-macroH2A1.2 ChIP libraries A and B in red.