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Table 2 Polymerase chain reaction results and clone sequencing from the stool specimen collected in this case report

From: Gut microeukaryotes during anorexia nervosa: a case report

Primers Total number of clones Number of clones per species Species Blast coverage % Blast coverage identity % Phylum
EUK1A/EUK516r 43 6/43 Mytilus trossulus (seefood) 100 99 Metazoan
   12/43 Lycium barbarum (tea) 100 98 Viridiplantae
   6/43 Juglans nigra (nut) 96 99 Viridiplantae
   8/43 Phaseoleae environmental 100 99 Viridiplantae
   2/43 Panax notoginseng (ginger) 100 99 Viridiplantae
   2/43 Fallopia japonica (infusion) 100 99 Viridiplantae
   3/43 Angelica gigas (infusion) 99 99 Viridiplantae
   2/43 Humulus lupulus 100 98 Viridiplantae
   1/43 Grevillea robusta 99 98 Viridiplantae
   1/43 Atractylodes japonica 100 99 Viridiplantae
JVF/DSPR2 60 24/60 Lycium barbarum 99 98 Viridiplantae
   9/60 Prunus persica (fruit) 96 99 Viridiplantae
   14/60 Phaseoleae environmental 99 99 Viridiplantae
   5/60 Carya glabra (nut) 96 99 Viridiplantae
   2/60 Saccharomyces cerevisiae 100 99 Fungi
   1/60 Laurus nobilis culinaire 99 99 Viridiplantae
   5/60 Mytilus galloprovincialis (seefood) 99 99 Metazoan
TFR1/TFR2 25 15/25 Tetratrichomonas sp. 100 100 Protozoan
   10/25 Humulus lupulus 100 98 Viridiplantae
MalF/MalR 56 34/56 Malassezia pachydermatis 100 93 Fungi
   12/56 Malassezia restricta 100 99 Fungi
   6/56 Malassezia globosa 100 99 Fungi
   4/56 Cystofilobasidium capitatum 100 99 Fungi
CuF/CUR 52 20/52 Trigonella foenum-graecummecinal (vegetables) 100 99 Viridiplantae
   8/52 Juglans regia (nut) 100 99 Viridiplantae
   19/52 Foeniculum vulgare (vegetables) 100 99 Viridiplantae
   5/52 Convolvulus arvensis (tea) 100 99 Viridiplantae
FunF/FunR 42 9/42 Dryas octopetala (tea) 99 99 Viridiplantae
   5/42 Penicillium solitum 99 99 Fungi
   4/42 Cladosporium bruhnei 100 99 Fungi
   2/42 Prunus persica (fruit) 99 98 Viridiplantae
   22/42 Sclerotium sp. 94 99 Fungi
rD5-ITS2/rb1-ITS2 70 70/70 Palaquium formosanum 100 93 Viridiplantae