Table 2 Polymerase chain reaction results and clone sequencing from the stool specimen collected in this case report
From: Gut microeukaryotes during anorexia nervosa: a case report
Primers | Total number of clones | Number of clones per species | Species | Blast coverage % | Blast coverage identity % | Phylum |
|---|---|---|---|---|---|---|
EUK1A/EUK516r | 43 | 6/43 | Mytilus trossulus (seefood) | 100 | 99 | Metazoan |
12/43 | Lycium barbarum (tea) | 100 | 98 | Viridiplantae | ||
6/43 | Juglans nigra (nut) | 96 | 99 | Viridiplantae | ||
8/43 | Phaseoleae environmental | 100 | 99 | Viridiplantae | ||
2/43 | Panax notoginseng (ginger) | 100 | 99 | Viridiplantae | ||
2/43 | Fallopia japonica (infusion) | 100 | 99 | Viridiplantae | ||
3/43 | Angelica gigas (infusion) | 99 | 99 | Viridiplantae | ||
2/43 | Humulus lupulus | 100 | 98 | Viridiplantae | ||
1/43 | Grevillea robusta | 99 | 98 | Viridiplantae | ||
1/43 | Atractylodes japonica | 100 | 99 | Viridiplantae | ||
JVF/DSPR2 | 60 | 24/60 | Lycium barbarum | 99 | 98 | Viridiplantae |
9/60 | Prunus persica (fruit) | 96 | 99 | Viridiplantae | ||
14/60 | Phaseoleae environmental | 99 | 99 | Viridiplantae | ||
5/60 | Carya glabra (nut) | 96 | 99 | Viridiplantae | ||
2/60 | Saccharomyces cerevisiae | 100 | 99 | Fungi | ||
1/60 | Laurus nobilis culinaire | 99 | 99 | Viridiplantae | ||
5/60 | Mytilus galloprovincialis (seefood) | 99 | 99 | Metazoan | ||
TFR1/TFR2 | 25 | 15/25 | Tetratrichomonas sp. | 100 | 100 | Protozoan |
10/25 | Humulus lupulus | 100 | 98 | Viridiplantae | ||
MalF/MalR | 56 | 34/56 | Malassezia pachydermatis | 100 | 93 | Fungi |
12/56 | Malassezia restricta | 100 | 99 | Fungi | ||
6/56 | Malassezia globosa | 100 | 99 | Fungi | ||
4/56 | Cystofilobasidium capitatum | 100 | 99 | Fungi | ||
CuF/CUR | 52 | 20/52 | Trigonella foenum-graecummecinal (vegetables) | 100 | 99 | Viridiplantae |
8/52 | Juglans regia (nut) | 100 | 99 | Viridiplantae | ||
19/52 | Foeniculum vulgare (vegetables) | 100 | 99 | Viridiplantae | ||
5/52 | Convolvulus arvensis (tea) | 100 | 99 | Viridiplantae | ||
FunF/FunR | 42 | 9/42 | Dryas octopetala (tea) | 99 | 99 | Viridiplantae |
5/42 | Penicillium solitum | 99 | 99 | Fungi | ||
4/42 | Cladosporium bruhnei | 100 | 99 | Fungi | ||
2/42 | Prunus persica (fruit) | 99 | 98 | Viridiplantae | ||
22/42 | Sclerotium sp. | 94 | 99 | Fungi | ||
rD5-ITS2/rb1-ITS2 | 70 | 70/70 | Palaquium formosanum | 100 | 93 | Viridiplantae |