Therapeutic use of a receptor mimic probiotic reduces intestinal Shiga toxin levels in a piglet model of hemolytic uremic syndrome

Table 1 Experimental design to assess the effect of a probiotic expressing a Shiga toxin receptor mimic on the incidence of edema disease

Experimental group	Number of pigs^a	Pig age at inoculation	Inoculation strain	Treatment
Probiotic	30	24-26 days	S1191^c	CWF308 (pJCPGb₄)
Sham	30	24-26 days	S1191^c	CWF308
Negative control	10	24-26 days	123^b	None

^aEach group represents the cumulative number of pigs from three separate experimental replications. ^bNon-pathogenic E. coli strain. ^cSTEC strain.
Three week old piglets were orally inoculated with either pathogenic STEC strain 1191 (probiotic and sham groups) or non-pathogenic E. coli strain 123 (negative control group). STEC inoculated pigs were treated with either a Shiga toxin 2e receptor mimic probiotic (probiotic group) or a sham probiotic strain which lacked the receptor mimic (sham group). Treatment was administered twice daily, and was initiated 24 hours post-inoculation with the STEC strain.

ISSN: 1756-0500