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Table 1 Phosphotriesterase kinetic parameters

From: Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase

  kcat(s−1) KM(μM) kcat/KM(M−1.s−1)
Paraoxon 0.12 ± 0.01 434 ± 54 2.81 (±0.38) × 102
Paraoxon 0.01% SDS 0.28 ± 0.01 537 ± 48 5.22 (±0.51) × 102
Paraoxon 0.1% SDS 0.25 ± 0.01 405 ± 21 6.10 (±0.34) × 102
Methyl Paraoxon 0.31 ± 0.02 278 ± 40 1.10 (±0.17) × 103
Parathion ND ND ND
Methyl Parathion ND ND 4.31 ± 0.20
Malathion ND ND ND
CMP-Coumarin 0.28 ± 0.02 642 ± 89 4.38 (±0.68) × 102
  1. ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.