Skip to main content

Table 1 Phosphotriesterase kinetic parameters

From: Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase

 

kcat(s−1)

KM(μM)

kcat/KM(M−1.s−1)

Paraoxon

0.12 ± 0.01

434 ± 54

2.81 (±0.38) × 102

Paraoxon 0.01% SDS

0.28 ± 0.01

537 ± 48

5.22 (±0.51) × 102

Paraoxon 0.1% SDS

0.25 ± 0.01

405 ± 21

6.10 (±0.34) × 102

Methyl Paraoxon

0.31 ± 0.02

278 ± 40

1.10 (±0.17) × 103

Parathion

ND

ND

ND

Methyl Parathion

ND

ND

4.31 ± 0.20

Malathion

ND

ND

ND

CMP-Coumarin

0.28 ± 0.02

642 ± 89

4.38 (±0.68) × 102

  1. ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.