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Table 2 Esterase kinetic parameters

From: Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase

 

kcat(s−1)

KM(μM)

kcat/KM(M−1.s−1)

Phenyl-acetate

0.35 ± 0.05

8 181 ± 1750

42.3 ± 11.1

p NP-acetate

0.13 ± 0.01

2 107 ± 313

60.1 ± 9.9

p NP-decanoate

ND

ND

ND

  1. ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.