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Table 3 Lactonase kinetic parameters

From: Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase

  kcat(s−1) KM(μM) kcat/KM(M−1.s−1)
C4 AHL ND ND ND
C6 AHL ND ND ND
C8 AHL 0.94 ± 0.02 178 ± 26 5.28 (±0.77) × 103
3-oxo C6 AHL ND ND ND
3-oxo C8 AHL 0.89 ± 0.07 836 ± 178 1.07 (±0.25) × 103
3-oxo C10 AHL 1.03 ± 0.04 213 ± 33 4.88 (±0.77) × 103
γ heptanolide 10.25 ± 0.50 388 ± 62 2.64 (±0.44) × 104
Nonanoic-γ-lactone 2.64 ± 0.07 109 ± 19 2.44 (±0.44) × 104
Undecanoic-γ-lactone 0.34 ± 0.01 578 ± 78 5.89 (±0.84) × 102
dodecanoic-γ-lactone 0.53 ± 0.03 242 ± 60 2.21 (±0.57) × 103
Nonanoic-δ-lactone 4.55 ± 0.21 348 ± 53 1.31 (±0.21) × 104
Undecanoic-δ-lactone 1.05 ± 0.05 168 ± 37 6.22 (±1.40) × 103
Dodecanoic-δ-lactone 3.34 ± 0.07 185 ± 27 1.81 (±0.27) × 104
ϵ caprolactone 15.04 ± 0.47 1 031 ± 83 1.46 (±0.13) × 104
Dihydrocoumarine ND ND ND
  1. ND correspond to Not Detected hydrolysis. Results have been obtained with cobalt as cofactor.