Table 3 Lactonase kinetic parameters
From: Sac Pox from the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius is a proficient lactonase
|  | kcat(s−1) | KM(μM) | kcat/KM(M−1.s−1) |
|---|---|---|---|
C4 AHL | ND | ND | ND |
C6 AHL | ND | ND | ND |
C8 AHL | 0.94 ± 0.02 | 178 ± 26 | 5.28 (±0.77) × 103 |
3-oxo C6 AHL | ND | ND | ND |
3-oxo C8 AHL | 0.89 ± 0.07 | 836 ± 178 | 1.07 (±0.25) × 103 |
3-oxo C10 AHL | 1.03 ± 0.04 | 213 ± 33 | 4.88 (±0.77) × 103 |
γ heptanolide | 10.25 ± 0.50 | 388 ± 62 | 2.64 (±0.44) × 104 |
Nonanoic-γ-lactone | 2.64 ± 0.07 | 109 ± 19 | 2.44 (±0.44) × 104 |
Undecanoic-γ-lactone | 0.34 ± 0.01 | 578 ± 78 | 5.89 (±0.84) × 102 |
dodecanoic-γ-lactone | 0.53 ± 0.03 | 242 ± 60 | 2.21 (±0.57) × 103 |
Nonanoic-δ-lactone | 4.55 ± 0.21 | 348 ± 53 | 1.31 (±0.21) × 104 |
Undecanoic-δ-lactone | 1.05 ± 0.05 | 168 ± 37 | 6.22 (±1.40) × 103 |
Dodecanoic-δ-lactone | 3.34 ± 0.07 | 185 ± 27 | 1.81 (±0.27) × 104 |
ϵ caprolactone | 15.04 ± 0.47 | 1 031 ± 83 | 1.46 (±0.13) × 104 |
Dihydrocoumarine | ND | ND | ND |