Skip to main content

Table 2 Assays used for the cross reactivity studies in adrenal extracts from dog

From: Chromogranins can be measured in samples from cats and dogs

Assay

MV

SD

CV

Suitable assay

Aa homology

CGA 17-38

3,02

0,26

8,8%

Yes

100%

CGA 176-195

0,10

0,02

15,5%

No

79%

CGA 238-247

0,24

0,11

47,0%

No

75%

CGA 324-337

1,41

0,18

12,7%

Yes

79%

CGA 361-372

0,46

0,02

3,6%

Yes

70%

SG2 154-165

7,22

0,48

6,7%

Yes

100%

CGB (Eurodiagnostica)

3,89

0,42

10,7%

Yes

77%

CGA (Eurodiagnostica)

4,99

1,22

24,5%

No

 
  1. The table shows the assays used for the cross reactivity studies of Chromogranin A (CGA), Chromogranin B (CGB) and Secretogranin II (SG2) measurements in adrenal extracts from dog. A coefficient of variation (CV = SD/MV) less than 13.5% (=1.5 x assay CV) was considered acceptable for measurements i.e. determined as a suitable assay, see text for explanation. The amino acid (Aa) homology between the human and canine species is indicated for the different assays.