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Table 2 Cycling condition for one-step RT-PCR

From: Mutation analysis of genes in the EGFR pathway in Head and Neck cancer patients: implications for anti-EGFR treatment response

Stage Temperature Time # cycles
Stage I preheating 95°C 1 min 1
Stage II: reverse transcription 60°C 30 min 1
Stage III: activation 95°C 10 min 1
Stage IV: cycling 95°C 15 sec 45
  60°C 1 min  
Stage V: denaturation 95°C 1 min  
Stage VI: melting 50°C-85°C Continues measuring  
Stage VII: cool 40°C 30 sec