Table 2 Cycling condition for one-step RT-PCR

Stage I preheating

95°C

1 min

1

Stage II: reverse transcription

60°C

30 min

1

Stage III: activation

95°C

10 min

1

Stage IV: cycling

95°C

15 sec

45

60°C

1 min

Stage V: denaturation

95°C

1 min

Stage VI: melting

50°C-85°C

Continues measuring

Stage VII: cool

40°C

30 sec