Table 2 Cycling condition for one-step RT-PCR
| Stage | Temperature | Time | # cycles |
|---|---|---|---|
| Stage I preheating | 95°C | 1 min | 1 |
| Stage II: reverse transcription | 60°C | 30 min | 1 |
| Stage III: activation | 95°C | 10 min | 1 |
| Stage IV: cycling | 95°C | 15 sec | 45 |
| 60°C | 1 min | ||
| Stage V: denaturation | 95°C | 1 min | |
| Stage VI: melting | 50°C-85°C | Continues measuring | |
| Stage VII: cool | 40°C | 30 sec |