Figure 2
From: Death associated protein kinase 2 is expressed in cortical interstitial cells of the mouse kidney
Cortical peritubular expression of DAPK2 in kidney. (A-C) Representative immunofluorescent images of DAPK2 staining (white arrows) of six wild type mice. Kidney sections from six Dapk2 null mice were used as specificity controls for the antiserum (insets A-C). Dashed circles indicate nonspecific staining in glomeruli. DAPK2 was localized to a population of interstitial cells that extend from (A) the renal cortex to (B) the cortical-medullary boundary. (C) No expression of DAPK2 was found in the inner medulla. (D) Western Blot analysis of tissue samples from regions of the kidney confirms expression of DAPK2 specifically in the cortex. (E) Immunofluorescence of DAPK2 using the same antibody in human kidney shows specific staining in proximal tubules (white arrows). Immunofluroescence images of DAPK2 staining at embryonic and postnatal stages (F) E15.5, (G) P0, (H) P3, and (I) P14. Expression is seen only at P14 (arrow). Scale bars = 50 μm.