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Figure 5 | BMC Research Notes

Figure 5

From: Death associated protein kinase 2 is expressed in cortical interstitial cells of the mouse kidney

Figure 5

Dapk2 null mice show resistance to fibrosis. In a chronic cisplatin induced model of CKD, 12 wild type and 10 mutant animals were analyzed for markers of fibrosis. Representative images show (A,B) reduced collagen deposition in the Dapk2 null as demonstrated by representative Masson’s trichrome staining (blue color indicated by arrows), and similar levels of (C,D) αSMA and (E,F) BrdU staining after treatment (arrows indicate positive cells, ‘a’ indicates arterial smooth muscle). (G,H) pSMAD2 staining after treatment showing equivalent numbers of positive interstitial nuclei, white arrows indicate positive pSMAD2 interstitial nuclei, open arrows indicate pSMAD2 negative nuclei. (I) Graph depicting the relative levels of staining between cisplatin treated of both genotypes normalized to untreated wild type values. Quantitative analysis is based on 100 fields in cisplatin treated mice. Dashed circles = glomeruli, scale bars = 50 μM.

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