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Table 2 Characterisation of four multiplex assays for Mustelus mustelus based on 87 individuals from southern Africa

From: Microsatellite cross-species amplification and utility in southern African elasmobranchs: A valuable resource for fisheries management and conservation

Locus Microsatellite repeat motif [P] Dye Size range (bp) A N A E H O H E PIC F IS Fr NULL P E-W
Mh1 (AG)n 0.2 VIC 191-211 7 2.2 0.885 0.544 0.443 -0.633** -0.223 0.931
Mh2 (GA)9 0.3 VIC 587-597 4 1.7 0.367 0.402 0.342 0.089** 0.023 0.688
Mh9 (GA)9 0.4 FAM 312-326 5 1.7 0.337 0.429 0.373 0.214** 0.062 b 0.723
Mh25 (CT)n 0.2 FAM 122-148 8 1.6 0.356 0.404 0.385 0.118** 0.032 0.802
Mca25 (CA)n(CT)n 0.2 PET 232-240 3 1.9 0.563 0.463 0.382 -0.217** -0.070 0.226
McaB39 (CA)10GAT(AC)8 0.2 NED 177-212 3 2.0 1.000 0.509 0.384 -0.977** -0.328 0.501
MPS1 (mean) - - - - 5 1.9 0.585 0.459 0.385 -0.234 -0.084 0.645
McaB5 (GT)11 0.2 VIC 189-210 10 3.5 0.826 0.716 0.674 -0.155* -0.067 0.330
McaB6 (CA)10 0.2 FAM 226-266 9 3.3 0.756 0.702 0.655 -0.077* -0.034 0.498
McaB22 (AC)18 0.2 NED 137-179 12 8.2 0.874 0.882 0.865 0.010 0.002 0.002
McaB27 (GT)6 0.2 PET 138-199 4 2.1 0.965 0.536 0.424 -0.808** -0.282 0.589
Mca33 (ATC)5 0.2 FAM 189-199 6 3.0 0.872 0.674 0.609 -0.295** -0.121 0.347
McaB37 (GT)5 0.2 NED 219-251 11 1.9 0.483 0.486 0.431 0.007** -0.016 0.997
MPS2 (mean) - - - - 9 3.7 0.796 0.666 0.610 -0.220 -0.086 0.461
Gg2 (TG)n 0.2 NED 249-259 7 3.2 1.000 0.688 0.632 -0.458** -0.188 0.324
Gg3 (GATT)n 0.2 PET 257-265 2 2.0 1.000 0.503 0.375 -1.000** -0.333 0.001
Gg7 (AG)n 0.2 NED 296-312 4 1.6 0.310 0.393 0.343 0.212** 0.058b 0.584
Gg11 (TCCC)n 0.2 NED 329-363 4 1.2 0.061 0.182 0.173 0.666** 0.000 0.792
Gg12 (TA)n 0.2 FAM 276-296 4 1.8 0.610 0.454 0.361 -0.347** -0.110 0.807
MPS3 (mean) - - - - 4.2 2.0 0.596 0.444 0.377 -0.185 -0.115 0.495
Gg15 (GA)n 0.2 FAM 147-169 3 2.05 0.977 0.514 0.392 -0.910** -0.308 0.370
Gg17 (AC)n 0.2 PET 159-181 3 1.02 0.023 0.023 0.023 -0.003** 0.000 1.000
Gg18 (GA)n 0.2 VIC 179-187 6 2.24 0.976 0.558 0.456 -0.759** -0.272 0.776
Gg22 (GT)n 0.2 FAM 237-247 4 2.25 0.964 0.559 0.455 -0.733** -0.263 0.488
Gg23 (AC)n 0.2 VIC 258-278 6 2.84 1.000 0.651 0.582 -0.540** -0.214 0.562
MPS4 (mean) - - - - 4.4 2.08 0.788 0.461 0.3816 -0.589 -0.211 0.615
Overall (mean) - - - - 5.7 2.4 0.691 0.512 0.444 0.010 -0.139 0.561
  1. Primer concentration in the final reaction as μM/primer ([P]); Number of alleles per locus (A N ); effective number of alleles (A E ); observed heterozygosity (H O ); expected heterozygosity (H E ); polymorphic information content (PIC); inbreeding coefficient (F IS ) with statistically significant deviations from Hardy-Weinberg expectations indicated by *(P < 0.01) and **(P < 0.001); null allele frequency (Fr NULL ) with bindicating the presence of null alleles at statistical significance at the 5% nominal level and Ewans-Watterson probability (P E-W ). Mean values for each multiplex assay and overall are indicated in bold.