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Table 2 Characterisation of four multiplex assays for Mustelus mustelus based on 87 individuals from southern Africa

From: Microsatellite cross-species amplification and utility in southern African elasmobranchs: A valuable resource for fisheries management and conservation

Locus

Microsatellite repeat motif

[P]

Dye

Size range (bp)

A N

A E

H O

H E

PIC

F IS

Fr NULL

P E-W

Mh1

(AG)n

0.2

VIC

191-211

7

2.2

0.885

0.544

0.443

-0.633**

-0.223

0.931

Mh2

(GA)9

0.3

VIC

587-597

4

1.7

0.367

0.402

0.342

0.089**

0.023

0.688

Mh9

(GA)9

0.4

FAM

312-326

5

1.7

0.337

0.429

0.373

0.214**

0.062 b

0.723

Mh25

(CT)n

0.2

FAM

122-148

8

1.6

0.356

0.404

0.385

0.118**

0.032

0.802

Mca25

(CA)n(CT)n

0.2

PET

232-240

3

1.9

0.563

0.463

0.382

-0.217**

-0.070

0.226

McaB39

(CA)10GAT(AC)8

0.2

NED

177-212

3

2.0

1.000

0.509

0.384

-0.977**

-0.328

0.501

MPS1 (mean)

-

-

-

-

5

1.9

0.585

0.459

0.385

-0.234

-0.084

0.645

McaB5

(GT)11

0.2

VIC

189-210

10

3.5

0.826

0.716

0.674

-0.155*

-0.067

0.330

McaB6

(CA)10

0.2

FAM

226-266

9

3.3

0.756

0.702

0.655

-0.077*

-0.034

0.498

McaB22

(AC)18

0.2

NED

137-179

12

8.2

0.874

0.882

0.865

0.010

0.002

0.002

McaB27

(GT)6

0.2

PET

138-199

4

2.1

0.965

0.536

0.424

-0.808**

-0.282

0.589

Mca33

(ATC)5

0.2

FAM

189-199

6

3.0

0.872

0.674

0.609

-0.295**

-0.121

0.347

McaB37

(GT)5

0.2

NED

219-251

11

1.9

0.483

0.486

0.431

0.007**

-0.016

0.997

MPS2 (mean)

-

-

-

-

9

3.7

0.796

0.666

0.610

-0.220

-0.086

0.461

Gg2

(TG)n

0.2

NED

249-259

7

3.2

1.000

0.688

0.632

-0.458**

-0.188

0.324

Gg3

(GATT)n

0.2

PET

257-265

2

2.0

1.000

0.503

0.375

-1.000**

-0.333

0.001

Gg7

(AG)n

0.2

NED

296-312

4

1.6

0.310

0.393

0.343

0.212**

0.058b

0.584

Gg11

(TCCC)n

0.2

NED

329-363

4

1.2

0.061

0.182

0.173

0.666**

0.000

0.792

Gg12

(TA)n

0.2

FAM

276-296

4

1.8

0.610

0.454

0.361

-0.347**

-0.110

0.807

MPS3 (mean)

-

-

-

-

4.2

2.0

0.596

0.444

0.377

-0.185

-0.115

0.495

Gg15

(GA)n

0.2

FAM

147-169

3

2.05

0.977

0.514

0.392

-0.910**

-0.308

0.370

Gg17

(AC)n

0.2

PET

159-181

3

1.02

0.023

0.023

0.023

-0.003**

0.000

1.000

Gg18

(GA)n

0.2

VIC

179-187

6

2.24

0.976

0.558

0.456

-0.759**

-0.272

0.776

Gg22

(GT)n

0.2

FAM

237-247

4

2.25

0.964

0.559

0.455

-0.733**

-0.263

0.488

Gg23

(AC)n

0.2

VIC

258-278

6

2.84

1.000

0.651

0.582

-0.540**

-0.214

0.562

MPS4 (mean)

-

-

-

-

4.4

2.08

0.788

0.461

0.3816

-0.589

-0.211

0.615

Overall (mean)

-

-

-

-

5.7

2.4

0.691

0.512

0.444

0.010

-0.139

0.561

  1. Primer concentration in the final reaction as μM/primer ([P]); Number of alleles per locus (A N ); effective number of alleles (A E ); observed heterozygosity (H O ); expected heterozygosity (H E ); polymorphic information content (PIC); inbreeding coefficient (F IS ) with statistically significant deviations from Hardy-Weinberg expectations indicated by *(P < 0.01) and **(P < 0.001); null allele frequency (Fr NULL ) with bindicating the presence of null alleles at statistical significance at the 5% nominal level and Ewans-Watterson probability (P E-W ). Mean values for each multiplex assay and overall are indicated in bold.
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BMC Research Notes

ISSN: 1756-0500

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