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Figure 1 | BMC Research Notes

Figure 1

From: Central role for protein kinase C in oxytocin and epidermal growth factor stimulated cyclooxygenase 2 expression in human myometrial cells

Figure 1

Mechanism of OXT induced COX-2 expression in human myometrial cells. Cells were stimulated with 1 μM OXT for 6 hours in the absence and in the presence of different inhibitors. The NFKB pathway was inhibited using TPCA-1 (2 μM). PD-184352 (2 μM) was used to test the ERK pathway. Signalling through PKC was inhibited by means of Bisindolylmaleimide I (BIS1; 5 μM). (A) Cell lysates were subjected to immunoblotting for COX-2. Blots were stripped and re-probed for GAPDH. (B) COX-2 expression was normalised to GAPDH expression and given as a percentage of the OXT treated value. Data points represent mean ± SEM, n = 5. Statistical significance was determined using one way ANOVA and Dunnett’s post hoc test. ***P < 0.001.

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