Figure 2

OXT stimulates ERK phosphorylation in primary human myometrial cells. Myometrial cells were stimulated with 1 μM OXT for different time points (2-, 5-, 10-, and 20-minutes) in the absence or presence of BIS1 (5 μM). (A) Cell lysates were subjected to immunoblotting using a phospho-ERK antibody. Blots were stripped and re-probed for ERK. (B) Fold change in ERK phosphorylation normalised to total ERK expression and non-treated values. Data points represent mean ± SEM, n = 3. Statistical significance was determined using two way ANOVA and Bonferroni post hoc test. **P < 0.01 and ***P < 0.001.