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Figure 3 | BMC Research Notes

Figure 3

From: Central role for protein kinase C in oxytocin and epidermal growth factor stimulated cyclooxygenase 2 expression in human myometrial cells

Figure 3

Mechanisms of EGF induced COX-2 expression in primary human myometrial cells. Cells were stimulated with 25 ng/ml EGF for 6 hours alone or in the presence of different inhibitors (2 μM TPCA-1, 2 μM PD-184352 and 5 μM BIS1). (A) Cell lysates were subjected to immunoblotting using a COX-2 antibody. Blots were stripped and re-probed for GAPDH. (B) COX-2 expression normalised to GAPDH expression and given as a percentage of the EGF treated value. Data points represent mean ± SEM, n = 3. Statistical significance was determined by means of one way ANOVA and Dunnett’s post hoc test. ** P < 0.01 and ***P < 0.001.

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