Figure 5From: Doppler ultrasound findings correlate with tissue vascularity and inflammation in surgical pathology specimens from patients with small intestinal Crohn’s diseaseHistopathological analysis of vascularity, inflammatory cell infiltration, and fibrosis. Tissue sections of “hyper-flow”, “hypo-flow”, and controls were stained with anti-factor VIII antibody, anti-CD163, CD3, CD79a MPO, or Masson’s trichrome. Positive-staining cells or areas were calculated using TissueMorph™. White, gray, and black bars represent control, “hypo-flow”, and “hyper-flow”, respectively. *p < 0.05. (A) The vascular walls were stained using an anti-factor VIII antibody, and the numbers of factor VIII-positive cells were calculated. (B) Inflammatory cell infiltrations were stained using anti-CD163, CD3, CD79a, and MPO antibody, and the numbers of positive-staining cells were calculated. (C) Fibrotic areas stained using Masson’s trichrome were evaluated, and the corresponding area was quantitated.Back to article page