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Table 1 Primers developed for Prosopis rubriflora and Prosopis ruscifolia

From: Characterization of microsatellite markers developed from Prosopis rubriflora and Prosopis ruscifolia (Leguminosae - Mimosoideae), legume species that are used as models for genetic diversity studies in Chaquenian areas under anthropization in South America

Marker

GenBank register no.

Primer sequences (5′-3′)

Motifs

Ta(°C)

Sizea(bp)

Crossed amplification

Prb1

KF923365

F: AACTACCGCAGCACTTTTCAGA

(gt)7

62.7

255-267

267b

R: ACTACTTGGAGATGCCGTGGA

Prb2

KF923366

F: GAAAGCCGCGCTCCTAAG

(gc)4(ac)7

61.0

140-146

126b

R: ATTCTTTTGTGTCTTGTCTTCTCG

Prb3

KF923367

F: TCCAAAGACCGCAAGAAGAT

(ca)7

61.0

149-159

145b

R: AGGCCAAAAAGGACTCAAAAT

Prb4

KF923368

F: ATCCGATAAATACACCTTCTGG

(ca)8

61.0

194-230

203b

R: GGTGTATCGTAAAAGCCTGG

Prb5

KF923369

F: TTTAAACATTGCACGTGAACCTAT

(ac)9

56.4

149-155

-

R: TTCACCCCTAAACCCCCTT

Prb6

KF923370

F: TCATCTCTCAAAGAAAACGCACTC

(tg)10

56.4

115-133

125b

R: CCGCAGAGAAGCCCCTACATA

Prb7

KF923371

F: GGCTTAGCATCACCCTCCAT

(ac)8

61.0

219-225

220b

R: CTTACCCTTTCAGTCCATTTACCA

Prb8

KF923372

F: CAACACCAAAACGGCGAGATGAT

(gt)13

61.0

144-164

154b

R: TTCGCCAAACGCCAGCATTAG

Prb9

KF923373

F: TTCTTCTCCTTCTTCATCTTCCTCC

(ac)9

62.7

167-175

190b

R: ACAACGTTGATCCCAAAACCTAAG

Prb10

KF923374

F: TTTTGGTGGATTTGATAGAGCC

(tca)5

56.4

223

-

R: GAGTGGGGTCAAGAAAGAACAG

Prsc1

KC753210

F: AATGGAGTTTGTTTGTGTCTGTGG

(ac)9(ct)5

56.5

279-297

300b

R: ATTACGGATACATCGAGCCTTCTT

Prsc2

KC753211

F: GCGGAATTCCAAACGACAA

(ac)9

64.7

224-252

250b

R: ACAGCAACACCCTCACTCTCAA

Prsc3

KC753212

F: CCACAAGCACACGCACACTCAGAC

(ca)6

64.7

156-160

122

R: CCAGCACTAGACTTCGCCACCAAC

Prsc4

KC753213

F: CAAAATCCAACAAATAAACACACC

(caa)2(ga)4

63.9

218-232

230b

R: GGCGGATTCTTGGCTCTCT

Prsc5

KC753214

F: CGCGTTAAGTCTGCCTTGCTTT

(gt)8

59.0

220-240

218

R: CTCATGGTATTTCCCTTGTCGTCC

Prsc6

KC753215

F: CGAGCGGCGAAAAATGATAAA

(gt)8

63.9

184-210

200

R: GCTGCTTCCCATAATCCTCTCCT

Prsc7

KC753216

F: CAGGGATTTAATCTCTTTGGTGTAG

(tg)8…(gtgg)2(gt)5

59.0

122-156

122b

R: ACAAGCTGGAAAGAGTCGCA

Prsc8

KC753217

F: AGTGACGTGAACACGCTGAGG

(tg)10

62.7

98-120

114b

R: TGCTGATGTGTGTGGTTTTGAGAT

Prsc9

KC753218

F: TCAGACTCCCGTGAACCAG

(tg)9

59.0

112-122

-

R: CGCACTCGAGCAGCATCT

Prsc10

KC753219

F: AACGCAACGGCCGCAACTAT

(ca)7(ct)7

56.5

260-284

-

R: ACAAAACGCTCGAATACTGGGGG

Prsc11

KC753220

F: CCCGGCAACTCAAATCAACTTCATA

(ac)11

62.7

229-371

244b

R: GGTCTAATTCTATTGGTGGGCTCTCTGG

Prsc12

KC753221

F: GGGGTGCATGTTGGGGATTG

(gt)10

59.0

185-223

220b

R: TTTGGCCGGATTAAAACAGAGCA

Prsc13

KC753222

F: CTTCACCATCACCGATTTCCCTT

(ctt)5

62.7

102

116

  

R: GCAACGAAGCAGCTGAAGAACAC

    
  1. Ta - Optimal annealing temperature defined after gradient tests of the corresponding markers. aRange of the fragment sizes from the polymorphic markers and the sequenced size of the monomorphic markers; bPolymorphism observed for the transferred markers based on the 5 samples used.