Exocytosis and processing of the Y-P30/dermcidin precursor. (A) Schematic of the constructs employed. (B + C) GFP-fusion constructs depicted in A were transfected into COS-7 and HEK-293 T cells. 24 hours after transfection the culture medium (supernatant) was collected and precipitated with 100% acetone to increase protein concentration. Cells were washed, harvested and lysed (pellet). Both, cell pellet and supernatant were analyzed by immunoblotting with anti-Y-P30 or anti-GFP antibodies. SP: signal peptide.