Western blot analysis. (A) The Y-P30/dermcidin precursor peptide is present in placenta at a molecular weight above 50 kDa (arrow). (B) With an ELISA assay we could also detect dermcidin in placenta extracts. Dermcidin levels in serum and plasma from the same patients were at the detection limit. (C + D) Y-P30/dermcidin precursor expression in placenta from normal pregnant women (INT, interruptio; TERM, after birth) and women suffering from pregnancy complications (SA, spontaneous abortion; PE, pre-eclampsia).