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Table 1 Boxes’ design and transport media

From: ‘All in a box’ a concept for optimizing microbiological diagnostic sampling in prosthetic joint infections

  Specimen type Bacteriological culture 16S rDNA sequencing FISH
Joint puncture Synovial fluid 15 mL tube (empty)* Tube B Tube C
Blood culture vial
Revision surgery Synovial fluid 15 mL tube (empty) Tube B Tube C
Soft tissue Tube A Tube D Tube C
Vials with Stuart transport medium (x5)*
Bone biopsies Tube A Tube D Tube C
Swabs from prosthesis*** Tube A Tube D Tube C
  Prosthesis (components or in toto) Empty container** Tube B** Tube C**
  1. Tube A: Modified Amies medium (Copan). Tube B: 2 mL tube with 60% glycerol in DEPC water, targeting a final concentration ≥10% glycerol including sample; estimated final concentration ~15-20%. Tube C: CyMol® (Copan). Tube D: Modified Amies medium with 20% glycerol (Copan). The transport media were 1] modified Amies medium for direct culture, 2] CyMol® for the preservation of nucleic acids for FISH, and 3] modified Amies medium with 20% glycerol (estimated final glycerol content ~10% incl. sample) for storage of whole bacterial cells at -80°C for subsequent molecular analyses (Copan). Stuart medium (SSI Diagnostica, Copenhagen Denmark) was used for biopsies of periprosthetic soft tissue obtained according to Kamme and Lindberg[7] and handled accordingly since the 1980’s.
  2. *Samples taken routinely during surgical revision.
  3. **The processing of prosthetic components took place in the laboratory (summarized in[2]). ***Swabs used to prosthetic scraping in situ for culture and 16S rDNA gene sequencing were CLASSIQSwab and for FISH (CyMol®) a FLOQSwab (Copan).