Figure 1From: Highly specific and efficient primers for in-house multiplex PCR detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticumRepresentative images of the standardisation process for the detection of C. trachomatis, N. gonorrhoeae, M. hominis and U. urealyticum by 16S rDNA gene amplification. Lanes MWM: 100-bp marker (Invitrogen™, Carlsbad, CA). The sizes (bp) are indicated on the left. The PCR products were electrophoresed on a 1.8% (wt/vol) agarose gel, stained with ethidium bromide and photographed under UV light. A. Lanes 1-4: Single PCR detection of M. hominis, C. trachomatis, N. gonorrhoeae and U. urealyticum. B. Lanes 1-2: Duplex PCR for the simultaneous detection of M. hominis and N. gonorrhoeae; lane 3: negative control (no template). C. Lanes 1-6: Triplex PCR for the simultaneous detection of M. hominis, N. gonorrhoeae and U. urealyticum; lane 7: negative control. D. Lanes 1-4: Quadruplex PCR for the simultaneous detection of M. hominis, N. gonorrhoeae, U. urealyticum and C. trachomatis; lane 5: negative control. Panels B, C and D also show the effects of the target DNA concentration on the multiplex amplifications; the best yield is shown in lanes 2, 6 and 4 using 100 ng of DNA template.Back to article page