Highly specific and efficient primers for in-house multiplex PCR detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum

Table 1 Evaluation of PCR primer analytical specificity

Inoculated microorganism	Analytical specificity results
	Addition of:		Amplification result in:
	2SP	Negative clinical sample^a	Single PCR	Quadruplex PCR
N. gonorrhoeae	Yes	Yes	+	+
G. vaginalis	Yes	Yes	-	-
L. acidophilus	Yes	Yes	-	-
None	Yes	Yes	-	-
Mix^b	Yes	Yes	-	-
S. agalactiae	Yes	No	-	-
N. gonorrhoeae	Yes	No	+	+
S. saprophyticus	Yes	No	-	-
None	Yes	No	-	-
Mix^b	Yes	No	-	-

+ = presence of amplicon.
- = no amplicon was visualised on agarose gel.
^aThe negativity of the samples was demonstrated by the detection of cervicovaginal pathogens using culture, Mycoplasma IST 2 – API galleries (bioMérieux, France) and/or PCR (data not shown).
^bThe mixes include G. vaginalis, L. acidophilus, S. agalactiae and S. saprophyticus.
2SP: 2-sucrose-phosphate transport medium.

ISSN: 1756-0500