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Table 1 Evaluation of PCR primer analytical specificity

From: Highly specific and efficient primers for in-house multiplex PCR detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum

Inoculated microorganism Analytical specificity results
Addition of: Amplification result in:
2SP Negative clinical samplea Single PCR Quadruplex PCR
N. gonorrhoeae Yes Yes + +
G. vaginalis Yes Yes - -
L. acidophilus Yes Yes - -
None Yes Yes - -
Mixb Yes Yes - -
S. agalactiae Yes No - -
N. gonorrhoeae Yes No + +
S. saprophyticus Yes No - -
None Yes No - -
Mixb Yes No - -
  1. + = presence of amplicon.
  2. - = no amplicon was visualised on agarose gel.
  3. aThe negativity of the samples was demonstrated by the detection of cervicovaginal pathogens using culture, Mycoplasma IST 2 – API galleries (bioMérieux, France) and/or PCR (data not shown).
  4. bThe mixes include G. vaginalis, L. acidophilus, S. agalactiae and S. saprophyticus.
  5. 2SP: 2-sucrose-phosphate transport medium.