Predicted structure and interaction sites of BIG3. Results of secondary structure prediction (PSIPRED) and interaction site prediction (PSIVER) were mapped on a structure-based alignment (generated with FUGUE and formatted with JOY ) between BIG3 (residues 86-300) and TIP120 (PDB 1u6g, chain C). For the PSIPRED prediction, H stands for α-helices, E for β strands and dash for coil structures. For the PSIVER results, scores greater than 0.39 are labelled with plus (+) signs. Clusters of the highest scoring residues are highlighted with a yellow background and those residues with a score greater than 0.6 are indicated with red plus signs. The helix used for the helical wheel projection (see Figure 4) is indicated with a red background. Structural environments are annotated with JOY, the formatting convention of which is as follows: red, α-helices; blue, β strands; maroon, 310 helices; upper case letters, solvent inaccessible; lower case letters, solvent accessible; bold type, hydrogen bonds to mainchain amides; underlining, hydrogen bonds to mainchain carbonyls; italic, positive mainchain torsion angles φ.