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Table 2 Search output produced by searching sheep-hamster PrP MS sequence data against a curated prion protein database (CD) alone and in conjunction with the public database, Swissprot

From: Fit-for-purpose curated database application in mass spectrometry-based targeted protein identification and validation

Sample

CDaonly

CD and Swissprot

 

Mascot score

Peptide identified

Mascot score

Peptide identified

SDS-PAGE gel band (replicate 1)

4117

12(11)¶

2232

12(10)

SDS-PAGE gel band (replicate 2)

2734

10(8)

1540

10(7)

  1. ¶Numbers without brackets denote total specific peptide match numbers while numbers in brackets denote significant specific peptide match numbers as per the Mascot search engine.