Skip to main content

Table 1 RNA quality and recovery

From: Identification of appropriate reference genes for qPCR studies in Staphylococcus pseudintermedius and preliminary assessment of icaA gene expression in biofilm-embedded bacteria

Sample ID

Concentration (ng/μL)

260/280

260/230

Total RNA recovery (μg)

RIN

A42 log A

616.2

2.15

1.09

61.6

9.6

A42 log B

771.4

2.15

2.27

77.1

n.t.

A42 log C

805.5

2.15

2.27

80.5

9.0

A54 log A

823.1

2.14

2.20

82.3

9.4

A54 log B

919.4

2.13

1.67

91.9

n.t.

A54 log C

921.6

2.14

2.03

92.2

9.4

A42 stat A

535.4

2.10

2.00

53.5

9.2

A42 stat B

449.4

2.02

1.95

44.9

n.t.

A42 stat C

493.0

2.05

1.66

49.3

8.8

A54 stat A

403.3

2.05

2.05

40.3

8.5

A54 stat B

630.1

2.13

1.89

63.0

n.t

A54 stat C

397.5

2.05

1.89

39.7

8.8

A42 BF A

732.4

2.12

2.17

36.6

9.4

A42 BF B

738.6

2.11

2.18

36.9

n.t

A42 BF C

887.3

2.12

2.19

44.4

8.4

A54 BF A

606.9

2.11

1.97

30.3

9.5

A54 BF B

337.9

2.06

1.74

16.9

n.t

A54 BF C

421.9

2.03

1.99

21.1

8.9

  1. (n.t: not tested, log: logarithmic, stat: stationary, BF: biofilm samples).